Mouse RAD50(DNA Repair Protein RAD50) ELISA Kit

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse DNA Repair Protein RAD50 (RAD50) in tissue homogenates, cell lysates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse DNA Repair Protein RAD50 (RAD50) in tissue homogenates, cell lysates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse DNA Repair Protein RAD50 (RAD50) in tissue homogenates, cell lysates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse DNA Repair Protein RAD50 (RAD50) in tissue homogenates, cell lysates and other biological fluids.

Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse DNA Repair Protein RAD50 (RAD50) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Description: tissue homogenates, cell lysates or other biological fluids.

Description: tissue homogenates, cell lysates or other biological fluids.

Description: tissue homogenates, cell lysates and other biological fluids.

Description: tissue homogenates, cell lysates and other biological fluids.

Description: tissue homogenates, cell lysates and other biological fluids.

Description: tissue homogenates, cell lysates and other biological fluids.

Description: A sandwich ELISA kit for detection of DNA Repair Protein RAD50 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Human DNA Repair Protein RAD50 (RAD50) in samples from tissue homogenates, cell lysates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Human DNA Repair Protein RAD50 (RAD50) in samples from tissue homogenates, cell lysates or other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human DNA Repair Protein RAD50 (RAD50) in Tissue homogenates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human DNA Repair Protein RAD50 (RAD50) in Tissue homogenates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human DNA Repair Protein RAD50 (RAD50) in Tissue homogenates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human DNA Repair Protein RAD50 (RAD50) in Tissue homogenates and other biological fluids.

Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human DNA Repair Protein RAD50 (RAD50) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Description: tissue homogenates, cell lysates or other biological fluids.

Description: tissue homogenates, cell lysates or other biological fluids.

Description: tissue homogenates, cell lysates and other biological fluids.

Description: tissue homogenates, cell lysates and other biological fluids.

Description: tissue homogenates, cell lysates and other biological fluids.

Description: tissue homogenates, cell lysates and other biological fluids.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RAD50. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RAD50. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RAD50, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RAD50 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RAD50. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RAD50. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RAD50, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RAD50 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: Mouse DNA Repair Protein RAD50 ELISA Kit

Description: A sandwich ELISA kit for detection of DNA Repair Protein RAD50 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Description: Recombinant Human DNA Repair Protein RAD50 expressed in: E.coli

Description: Recombinant Human DNA Repair Protein RAD50 expressed in: E.coli

Description: Recombinant Mouse DNA Repair Protein RAD50 expressed in: E.coli

Description: A Rabbit polyclonal antibody against Mouse DNA Repair Protein RAD50 (RAD50)

Description: A Rabbit polyclonal antibody against Mouse DNA Repair Protein RAD50 (RAD50). This antibody is labeled with PE.

Description: A Rabbit polyclonal antibody against Mouse DNA Repair Protein RAD50 (RAD50). This antibody is labeled with APC.

Description: A Rabbit polyclonal antibody against Mouse DNA Repair Protein RAD50 (RAD50). This antibody is labeled with Cy3.

Description: A Rabbit polyclonal antibody against Mouse DNA Repair Protein RAD50 (RAD50). This antibody is labeled with HRP.

Description: A Rabbit polyclonal antibody against Mouse DNA Repair Protein RAD50 (RAD50). This antibody is labeled with FITC.

Description: A Rabbit polyclonal antibody against Mouse DNA Repair Protein RAD50 (RAD50). This antibody is labeled with Biotin.

Description: Human DNA Repair Protein RAD50 ELISA Kit

Description: A Rabbit polyclonal antibody against Mouse DNA Repair Protein RAD50 (RAD50). This antibody is labeled with APC-Cy7.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50)

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50)

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with PE.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with PE.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with APC.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with Cy3.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with HRP.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with APC.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with Cy3.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with HRP.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with FITC.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with FITC.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with Biotin.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with Biotin.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with APC-Cy7.

Description: A Rabbit polyclonal antibody against Human DNA Repair Protein RAD50 (RAD50). This antibody is labeled with APC-Cy7.

Description: This Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD51.

Description: This Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD51.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RAD51B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RAD51B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RAD51B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RAD51B in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RAD51B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RAD51B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RAD51B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RAD51B in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: Quantitative sandwich ELISA for measuring Human DNA repair protein RAD51 homolog 1 (RAD51) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Description: Quantitative sandwich ELISA for measuring Human DNA repair protein RAD51 homolog 1 (RAD51) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Description: Quantitative sandwich ELISA for measuring Human DNA repair protein RAD51 homolog 1 (RAD51) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Description: Recombinant Human DNA repair protein RAD51 homolog 4(RAD51D) expressed in E.coli

Description: This Human UV excision repair protein RAD23 homolog B (RAD23B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD23B.

Description: This Human UV excision repair protein RAD23 homolog B (RAD23B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD23B.

Description: Recombinant Saccharomyces cerevisiae DNA repair and recombination protein RAD52(RAD52),partial expressed in E.coli

Description: Quantitative sandwich ELISA for measuring Human UV excision repair protein RAD23 homolog B (RAD23B) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Description: Quantitative sandwich ELISA for measuring Human UV excision repair protein RAD23 homolog B (RAD23B) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Description: Quantitative sandwich ELISA for measuring Human UV excision repair protein RAD23 homolog B (RAD23B) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Description: Description of target: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation (By similarity).By similarity2 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.4"Disruption of mRad50 causes embryonic stem cell lethality, abnormal embryonic development, and sensitivity to ionizing radiation."_x005F_x005F_x000D_Luo G., Yao M.S., Bender C.F., Mills M., Bladl A.R., Bradley A., Petrini J.H.J._x005F_x005F_x000D_Proc. Natl. Acad. Sci. U.S.A. 96:7376-7381(1999) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, DISRUPTION PHENOTYPE.Ref.5"Cancer predisposition and hematopoietic failure in Rad50(S/S) mice."_x005F_x005F_x000D_Bender C.F., Sikes M.L., Sullivan R., Huye L.E., Le Beau M.M., Roth D.B., Mirzoeva O.K., Oltz E.M., Petrini J.H.J._x005F_x005F_x000D_Genes Dev. 16:2237-2251(2002) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, MUTAGENESIS OF LYS-22. ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.059 ng/mL

Description: Recombinant Human UV excision repair protein RAD23 homolog A(RAD23A) expressed in Yeast

Description: Recombinant Human UV excision repair protein RAD23 homolog A(RAD23A) expressed in E.coli

Description: Available in various conjugation types.

Description: Description of target: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.;Species reactivity: Human;Application: ;Assay info: Quantitative Sandwich ELISA;Sensitivity: < 0.057 ng/mL

Description: Description of target: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point (PubMed:11741547, PubMed:9590181, PubMed:9705271, PubMed:9651580). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).6 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.7"The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response."_x005F_x005F_x000D_Carney J.P., Maser R.S., Olivares H., Davis E.M., Le Beau M., Yates J.R. III, Hays L., Morgan W.F., Petrini J.H.J._x005F_x005F_x000D_Cell 93:477-486(1998) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN DSB REPAIR, IDENTIFICATION IN THE MRN COMPLEX WITH MRE11A AND NBN.Ref.8"Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95."_x005F_x005F_x000D_Trujillo K.M., Yuan S.-S.F., Lee E.Y.-H.P., Sung P._x005F_x005F_x000D_J. Biol. Chem. 273:21447-21450(1998) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN DSB REPAIR, IDENTIFICATION IN THE MRN COMPLEX WITH MRE11A AND NBN.Ref.9"The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks."_x005F_x005F_x000D_Paull T.T., Gellert M._x005F_x005F_x000D_Mol. Cell 1:969-979(1998) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, ATP-BINDING, MUTAGENESIS OF LYS-42 AND ASP-1231.Ref.13"Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres."_x005F_x005F_x000D_Zhu X.-D., Kuester B., Mann M., Petrini J.H.J., de Lange T._x005F_x005F_x000D_Nat. Genet. 25:347-352(2000) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN TELOMERES, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE A COMPLEX WITH TERF2, SUBCELLULAR LOCATION.Ref.15"Human Rad50/Mre11 is a flexible complex that can tether DNA ends."_x005F_x005F_x000D_de Jager M., van Noort J., van Gent D.C., Dekker C., Kanaar R., Wyman C._x005F_x005F_x000D_Mol. Cell 8:1129-1135(2001) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTRAMOLECULAR COILED-COIL DOMAINS.Ref.19"Direct activation of the ATM protein kinase by the Mre11/Rad50/Nbs1 complex."_x005F_x005F_x000D_Lee J.-H., Paull T.T._x005F_x005F_x000D_Science 304:93-96(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN ATM ACTIVATION. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.057 ng/mL

Description: Mouse monoclonal Rad50 antibody

Description: Rabbit polyclonal RAD50 antibody

Description: Purified Polyclonal RAD50 antibody

Description: A polyclonal antibody against RAD50. Recognizes RAD50 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000

Description: A polyclonal antibody against RAD50. Recognizes RAD50 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:50-1:200

Description: A polyclonal antibody against RAD50. Recognizes RAD50 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:10000, WB:1:1000-1:5000

Description: A polyclonal antibody against RAD50. Recognizes RAD50 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000