Single-Cell Technologies Applied to HIV-1 Research: Reaching Maturity.

The want for definitive solutions in all probability explains our pure tendency to search simplicity. The reductionist “bulk” strategy, through which a imply habits is attributed to a heterogeneous cell inhabitants, fulfills this want by significantly serving to the conceptualization of complicated organic processes. However, the bounds of this system have gotten more and more clear as fashions search to clarify organic occasions occurring in vivo, the place heterogeneity is the rule.
Research within the HIV-1 subject is not any exception: the challenges encountered within the improvement of preventive and healing anti-HIV-1 methods could nicely originate partially from insufficient assumptions constructed on bulk applied sciences, highlighting the necessity for brand spanking new views.
The emergence of various single-cell applied sciences set the stage for potential breakthrough discoveries, as heterogeneous processes can now be investigated with an unprecedented depth in matters as various as HIV-1 tropism, dynamics of the replication cycle, latency, viral reservoirs and immune management. In this overview, we summarize latest advances within the HIV-1 subject made doable by single-cell applied sciences, and contextualize their significance.

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